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Addgene

pBHRSF228
(Plasmid #135007)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135007 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSF
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MusF2 prenyltransferase
  • Species
    Nostoc sp. UHCC 0398

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer SUMO fw
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

It was published as Addgene ID # 135007

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBHRSF228 was a gift from Hideo Iwai (Addgene plasmid # 135007 ; http://n2t.net/addgene:135007 ; RRID:Addgene_135007)

  • For your References section:

    Biosynthesis of the Bis-Prenylated Alkaloids Muscoride A and B. Mattila A, Andsten RM, Jumppanen M, Assante M, Jokela J, Wahlsten M, Mikula KM, Sigindere C, Kwak DH, Gugger M, Koskela H, Sivonen K, Liu X, Yli-Kauhaluoma J, Iwai H, Fewer DP. ACS Chem Biol. 2019 Dec 20;14(12):2683-2690. doi: 10.1021/acschembio.9b00620. Epub 2019 Nov 19. 10.1021/acschembio.9b00620 PubMed 31674754
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