pET28a-MBP-CBX1-eReader
(Plasmid #135089)

• Purpose: Bacterial expression of engineered CBX1 with enhanced binding to H3K9me
• Depositing Lab: Marcey Waters
• Publication: Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111. doi: 10.1021/acschembio.9b00651. Epub 2019 Nov 1.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Total vector size (bp): 6615
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CBX1 chromobox protein 1, engineered
• Alt name: CBX1 eR
• Species: H. sapiens (human)
• Insert Size (bp): 189
• Mutation: lysine 43 mutated to alanine; aspartic acid 59 mutated to phenylalanine
• Promoter: T7
• Tags / Fusion Proteins:
  • MBP (N terminal on backbone)
  • 6xhis-tag (N terminal on backbone)
  • 6xhis-tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry