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pDctp-mSox10-Mp1
(Plasmid #13513)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13513 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II SK(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sox10
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    6200
  • GenBank ID
    AF017182
  • Entrez Gene
    Sox10 (a.k.a. Dom, Sox21, gt)
  • Tags / Fusion Proteins
    • Dct promoter (N terminal on insert)
    • Mp1-polyA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer don't know
  • 3′ sequencing primer don't know
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pMG1, Dr. Glenn Merlino, NCI, NIH.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dct promoter sequence in Dr. Merlino's plasmid, published
by Dr. Ian Jackson, 1995

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDctp-mSox10-Mp1 was a gift from William Pavan (Addgene plasmid # 13513 ; http://n2t.net/addgene:13513 ; RRID:Addgene_13513)
  • For your References section:

    Genetic evidence does not support direct regulation of EDNRB by SOX10 in migratory neural crest and the melanocyte lineage. Hakami RM, Hou L, Baxter LL, Loftus SK, Southard-Smith EM, Incao A, Cheng J, Pavan WJ. Mech Dev. 2006 Feb . 123(2):124-34. 10.1016/j.mod.2005.11.004 PubMed 16412618