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pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP
(Plasmid #135176)


Item Catalog # Description Quantity Price (USD)
Plasmid 135176 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP
  • Backbone manufacturer
    addgene Plasmid #35509
  • Modifications to backbone
    Ef1a promoter was exchanged to TRE3G promoter obtained from pTRE3G-ZsGreen1 from Clontech.
  • Vector type
    Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Alt name
  • Species
    Chlamydomonas reinhardtii
  • Mutation
    E123T and T159C
  • Promoter TRE3G
  • Tag / Fusion Protein
    • EYFP (C terminal on insert) (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Species

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was modified from addgene plasmid #35509. EF1a promoter of #35509 was replaced by TRE3G promoter of pTRE3G-ZsGreen1 from Clontech. The use of TRE3G is licensed from TETsystems Inc.

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TRE-flex-ChR2 (E123T/T159C)-EYFP was a gift from Tetsuo Yamamori (Addgene plasmid # 135176 ; ; RRID:Addgene_135176)
  • For your References section:

    Arm movements induced by noninvasive optogenetic stimulation of the motor cortex in the common marmoset. Ebina T, Obara K, Watakabe A, Masamizu Y, Terada SI, Matoba R, Takaji M, Hatanaka N, Nambu A, Mizukami H, Yamamori T, Matsuzaki M. Proc Natl Acad Sci U S A. 2019 Nov 5;116(45):22844-22850. doi: 10.1073/pnas.1903445116. Epub 2019 Oct 21. 10.1073/pnas.1903445116 PubMed 31636197