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pAAV-TRE-flex-Clover
(Plasmid #135177)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 135177 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Stratagene
  • Modifications to backbone
    The promoter is replaced by TRE3 promoter of pTRE3G-ZsGreen1.
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Clover
  • Species
    Aequorea victoria
  • Mutation
    a variant of GFP
  • Promoter TRE3G

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    TRE3G
  • Species
    Synthetic

Cloning Information for Gene/Insert 2

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Clover was obtained from addgene plasmid #40259. TRE3G promoter was obtained from pTRE3G-ZsGreen1 of Clontech. The use of TRE3G is licensed from TETsystems Inc.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TRE-flex-Clover was a gift from Tetsuo Yamamori (Addgene plasmid # 135177 ; http://n2t.net/addgene:135177 ; RRID:Addgene_135177)
  • For your References section:

    Arm movements induced by noninvasive optogenetic stimulation of the motor cortex in the common marmoset. Ebina T, Obara K, Watakabe A, Masamizu Y, Terada SI, Matoba R, Takaji M, Hatanaka N, Nambu A, Mizukami H, Yamamori T, Matsuzaki M. Proc Natl Acad Sci U S A. 2019 Nov 5;116(45):22844-22850. doi: 10.1073/pnas.1903445116. Epub 2019 Oct 21. 10.1073/pnas.1903445116 PubMed 31636197