Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pAAV-R26-FLEX-mStr
(Plasmid #135619)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 135619 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Addgene 61591
  • Backbone size w/o insert (bp) 2894
  • Total vector size (bp) 7213
  • Vector type
    Mouse Targeting, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CAG-FLEX-mStrawberry flanked by Rosa26 homology arms
  • Alt name
    Rosa26
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4319
  • GenBank ID
    14910
  • Entrez Gene
    Gt(ROSA)26Sor (a.k.a. AV258896, Gtrgeo, Gtrgeo26, Gtro, Gtrosa26, R2, R26, RO, ROSA26, Thumpd3, Thumpd3as1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NsiI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-R26-FLEX-mStr was a gift from Lin He (Addgene plasmid # 135619 ; http://n2t.net/addgene:135619 ; RRID:Addgene_135619)
  • For your References section:

    CRISPR-READI: Efficient Generation of Knockin Mice by CRISPR RNP Electroporation and AAV Donor Infection. Chen S, Sun S, Moonen D, Lee C, Lee AY, Schaffer DV, He L. Cell Rep. 2019 Jun 25;27(13):3780-3789.e4. doi: 10.1016/j.celrep.2019.05.103. 10.1016/j.celrep.2019.05.103 PubMed 31242412