pAAV-R26-FLEX-mStr
(Plasmid #135619)

• Purpose: Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus
• Depositing Lab: Lin He
• Publication: Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e4. doi: 10.1016/j.celrep.2019.05.103.

Backbone

• Vector backbone: pAAV
• Backbone manufacturer: Addgene 61591
• Backbone size w/o insert (bp): 2894
• Total vector size (bp): 7213
• Vector type: Mouse Targeting, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CAG-FLEX-mStrawberry flanked by Rosa26 homology arms
• Alt name: Rosa26
• Species: M. musculus (mouse)
• Insert Size (bp): 4319
• GenBank ID: 14910
• Entrez Gene: Gt(ROSA)26Sor (a.k.a. Gtrgeo26, Gtrosa26, R26, ROSA26, Thumpd3as1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NsiI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pAAV-R26-FLEX-mStr.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry