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pTAHR TH-p2a-TD:Tomato (floxed selection Puro)
(Plasmid #135814)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 135814 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    HR120PA-1
  • Backbone manufacturer
    SBI
  • Backbone size w/o insert (bp) 7672
  • Total vector size (bp) 9204
  • Modifications to backbone
    removed GFP fluorescent cassette inserted p2aTdTomato WPRE cassette via assembly, added 5'TH and 3'TH homology arms via assembly
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression.
  • Alt name
    last exon of human TH gene
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_000360.4
  • Entrez Gene
    TH (a.k.a. DYT14, DYT5b, TYH)
  • Tag / Fusion Protein
    • p2a-TdTomato

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAHR TH-p2a-TD:Tomato (floxed selection Puro) was a gift from Tim Ahfeldt & Lee Rubin (Addgene plasmid # 135814 ; http://n2t.net/addgene:135814 ; RRID:Addgene_135814)
  • For your References section:

    Pathogenic Pathways in Early-Onset Autosomal Recessive Parkinson's Disease Discovered Using Isogenic Human Dopaminergic Neurons. Ahfeldt T, Ordureau A, Bell C, Sarrafha L, Sun C, Piccinotti S, Grass T, Parfitt GM, Paulo JA, Yanagawa F, Uozumi T, Kiyota Y, Harper JW, Rubin LL. Stem Cell Reports. 2019 Dec 20. pii: S2213-6711(19)30444-8. doi: 10.1016/j.stemcr.2019.12.005. 10.1016/j.stemcr.2019.12.005 PubMed 31902706