pTACR TH2-p2aGFP
(Plasmid
#135816)
-
PurposeCRISPR based on px330 p2aGFP targeting TH gene near stop codon
-
Depositing Labs
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135816 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepx330
- Backbone size w/o insert (bp) 8484
- Total vector size (bp) 9405
-
Modifications to backboneadded p2a MYR eGFP cloned TH spacer into BBSI site
-
Vector typeCRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTH 2 guide
-
Tag
/ Fusion Protein
- p2a Myr-eGFP
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTACR TH2-p2aGFP was a gift from Tim Ahfeldt & Lee Rubin (Addgene plasmid # 135816 ; http://n2t.net/addgene:135816 ; RRID:Addgene_135816) -
For your References section:
Pathogenic Pathways in Early-Onset Autosomal Recessive Parkinson's Disease Discovered Using Isogenic Human Dopaminergic Neurons. Ahfeldt T, Ordureau A, Bell C, Sarrafha L, Sun C, Piccinotti S, Grass T, Parfitt GM, Paulo JA, Yanagawa F, Uozumi T, Kiyota Y, Harper JW, Rubin LL. Stem Cell Reports. 2019 Dec 20. pii: S2213-6711(19)30444-8. doi: 10.1016/j.stemcr.2019.12.005. 10.1016/j.stemcr.2019.12.005 PubMed 31902706
Map uploaded by the depositor.
