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pSPL3-ABCC8-27
(Plasmid #135919)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135919 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 135919-D50 50 μg of DNA in Tris buffer $495
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DNA 135919-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pSPL3
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 6031
  • Total vector size (bp) 5423
  • Modifications to backbone
    We cut out 943 bp and inserted 353 from ABCC8
  • Vector type
    Mammalian Expression ; Exon trapping

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATP-binding Cassette subfamily C member 8
  • Alt name
    ABCC8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    351
  • GenBank ID
    NM_001351296.2
  • Entrez Gene
    ABCC8 (a.k.a. ABC36, HHF1, HI, HRINS, MRP8, PHHI, PNDM3, SUR, SUR1, SUR1delta2, TNDM2)
  • Promoter SV40

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CTCGAGAAATTGGCAGAGGATGCCAGA
  • 3′ sequencing primer GCTAGCTAATCGGATCGGGGACACTG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSPL3-ABCC8-27 was a gift from Diana Valverde Pérez (Addgene plasmid # 135919 ; http://n2t.net/addgene:135919 ; RRID:Addgene_135919)

  • For your References section:

    Characterization of rare ABCC8 variants identified in Spanish pulmonary arterial hypertension patients. Lago-Docampo M, Tenorio J, Hernandez-Gonzalez I, Perez-Olivares C, Escribano-Subias P, Pousada G, Baloira A, Arenas M, Lapunzina P, Valverde D. Sci Rep. 2020 Sep 15;10(1):15135. doi: 10.1038/s41598-020-72089-1. 10.1038/s41598-020-72089-1 PubMed 32934261
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