EGFP-Mm_MyoGEF(WT)
(Plasmid
#136325)
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PurposeMammalian expression of mouse MyoGEF-WT with N-terminal EGFP
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Depositing Labs
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Depositing OrganizationCentro Nacional de Investigaciones Oncologicas (CNIO)
Located in Spain -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136325 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 136325-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 136325-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCDNA3.1 DEST_GFP
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePLEKHG6
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Alt nameMyoGEF
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SpeciesM. musculus (mouse)
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Entrez GenePlekhg6 (a.k.a. Gm155, BC026778, MGC30363)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 136325-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 136325-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-Mm_MyoGEF(WT) was a gift from Guillermo de Cárcer & Marcos Malumbres (Addgene plasmid # 136325 ; http://n2t.net/addgene:136325 ; RRID:Addgene_136325) -
For your References section:
Plk1 regulates contraction of postmitotic smooth muscle cells and is required for vascular homeostasis. de Carcer G, Wachowicz P, Martinez-Martinez S, Oller J, Mendez-Barbero N, Escobar B, Gonzalez-Loyola A, Takaki T, El Bakkali A, Camara JA, Jimenez-Borreguero LJ, Bustelo XR, Canamero M, Mulero F, de Los Angeles Sevilla M, Montero MJ, Redondo JM, Malumbres M. Nat Med. 2017 Aug;23(8):964-974. doi: 10.1038/nm.4364. Epub 2017 Jul 10. 10.1038/nm.4364 PubMed 28692064
