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pYPQ239A (dFnCas12a)-TV
(Plasmid #136379)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 136379 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dFnCas12a-TV
  • Species
    Francisella novicida U112
  • Mutation
    D917A

Resource Information

Depositor Comments

TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-0

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ239A (dFnCas12a)-TV was a gift from Yiping Qi (Addgene plasmid # 136379 ; http://n2t.net/addgene:136379 ; RRID:Addgene_136379)
  • For your References section:

    CRISPR–Cas12b enables efficient plant genome engineering. Ming M, Ren Q, Pan C, He Y, Zhang Y, Liu S, Zhong Z, Wang J, Malzahn AA, Wu J, Zheng X, Zhang Y, and Qi Y.. Nature Plants (2020) 10.1038/s41477-020-0614-6