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CRISPR–Cas12b enables efficient plant genome engineering
Ming M, Ren Q, Pan C, He Y, Zhang Y, Liu S, Zhong Z, Wang J, Malzahn AA, Wu J, Zheng X, Zhang Y, and Qi Y.
Nature Plants (2020)
Article

Plasmids from Article

ID Plasmid Purpose  
129670pYPQ290Gateway entry clone for CRISPR-Cas12b systems
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129671pYPQ291Gateway entry clone for CRISPR-Cas12b systems
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129672pYPQ292Gateway entry clone for CRISPR-Cas12b systems
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129673pYPQ290-D570AGateway entry clone for CRISPR-Cas12b systems
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129674pYPQ290-D977AGateway entry clone for CRISPR-Cas12b systems
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129675pYPQ290-E848AGateway entry clone for CRISPR-Cas12b systems
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129676pYPQ291-D573AGateway entry clone for CRISPR-Cas12b systems
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129677pYPQ291-D951AGateway entry clone for CRISPR-Cas12b systems
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129678pYPQ291-E827AGateway entry clone for CRISPR-Cas12b systems
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129679pYPQ292-D570AGateway entry clone for CRISPR-Cas12b systems
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129680pYPQ292-D977AGateway entry clone for CRISPR-Cas12b systems
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129681pYPQ292-E848AGateway entry clone for CRISPR-Cas12b systems
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129682pYPQ290-D570A-SRDXGateway entry clone for CRISPR-Cas12b systems
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129683pYPQ291-D573A-SRDXGateway entry clone for CRISPR-Cas12b systems
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129684pYPQ292-D570A-SRDXGateway entry clone for CRISPR-Cas12b systems
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129685pYPQ141-ZmUbi-RZ-Aac(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter
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129686pYPQ141-ZmUbi-RZ-Bth(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter
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136372pYPQ141-ZmUbi-RZ-Aa1.2.3Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is used
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136373pYPQ141-ZmUbi-RZ-Aa1.2Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 is used
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136374pYPQ141-ZmUbi-RZ-Aa3.8.3Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used
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136375pYPQ141-ZmUbi-RZ-Aa3.8.4Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used
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136376pYPQ141-ZmUbi-RZ-Aa3.8Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is used
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136377pYPQ141-ZmUbi-RZ-Aac.3Gateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is used
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136378pYPQ141-ZmUbi-RZ-BhGateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processing
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136379pYPQ239A (dFnCas12a)-TVCRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system
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136380pYPQ292 (AaCas12b)-D570A-TVCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system
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136381pYPQ292 (AaCas12b)-D570A-TV-MS2-TVCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein
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136382pYPQ292 (AaCas12b)-D570A-TV-MS2-VPRCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein
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136383pYPQ293 (BhCas12b_v4)Gateway entry clone for BhCas12b_v4
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