|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||136382||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typePlant Expression, CRISPR
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAaCas12b (D570A)-TV-T2A-MCP-VPR
TV activation system is from: Li, Z., Zhang, D., Xiong, X. et al. A potent Cas9-derived gene activator for plant and mammalian cells. Nature Plants 3, 930–936 (2017) doi:10.1038/s41477-017-0046-3; VPR activation system is from: Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, E PRI, et al. Highly efficient Cas9-mediated transcriptional programming. Nat Methods. 2015;12(4):326–8. 10.1038/nmeth.3312
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR was a gift from Yiping Qi (Addgene plasmid # 136382 ; http://n2t.net/addgene:136382 ; RRID:Addgene_136382)
For your References section:CRISPR–Cas12b enables efficient plant genome engineering. Ming M, Ren Q, Pan C, He Y, Zhang Y, Liu S, Zhong Z, Wang J, Malzahn AA, Wu J, Zheng X, Zhang Y, and Qi Y.. Nature Plants (2020) 10.1038/s41477-020-0614-6