pKD072
(Plasmid #136472)

• Purpose: SSB-C-term-GFP fusion expression plasmid
• Depositing Lab: James Keck
• Publication: Development of a single-stranded DNA-binding protein fluorescent fusion toolbox (unpublished)

Backbone

• Vector backbone: pET21a
• Backbone size w/o insert (bp): 5382
• Total vector size (bp): 6639
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SSB-C-term-GFP
• Species: E. coli
• Insert Size (bp): 1257
• Mutation: superfolder GFP attached to C-terminus at Phe 178
• GenBank ID: NC_000913.3
• Entrez Gene: ssb (a.k.a. b4059, ECK4051, exrB, lexC)
• Promoter: T7 promoter
• Tag / Fusion Protein:
  • superfolder GFP (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TAA TAC GAC TCA CTA TAG GG
• 3′ sequencing primer: GCT AGT TAT TGC TCA GCG G

Resource Information

• Supplemental Documents:
  • pKD072.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry