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pB-actin 16 E6*
(Plasmid #13714)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 13714 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 13714-D50 50 μg of DNA in Tris buffer $495
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DNA 13714-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pB-actin
  • Backbone size w/o insert (bp) 7500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HPV 16 E6*
  • Alt name
    HPV 16 E6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    338
  • Mutation
    del bp 128-311 and bp 352-473 of coding region
  • GenBank ID
    NC_001526
  • Entrez Gene
    E6 (a.k.a. HpV16gp1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal 1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Schneider-Gadicke A., Schwarz E. EMBO, vol. 5, no. 9, 2285-2292.

Addgene NGS results found that the E6* protein sequence lacks the first 7 amino acids compared to the NCBI reference [YP_009268707.1].

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB-actin 16 E6* was a gift from Karl Munger (Addgene plasmid # 13714 ; http://n2t.net/addgene:13714 ; RRID:Addgene_13714)

  • For your References section:

    The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. Münger K, Phelps WC, Bubb V, Howley PM, Schlegel R. J Virol. 1989 Oct . 63(10):4417-21. PubMed 2476573
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