pAAV-Ef1a-Con/Fon/Von GCaMP 6m
(Plasmid
#137165)
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PurposeIntersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 137165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAAV2-Ef1a-WPRE
- Backbone size w/o insert (bp) 4600
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Vector typeAAV, Cre/Lox, Synthetic Biology ; Flp/FRT
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3x-GCaMP6M
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SpeciesSynthetic; Prokaryotic
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Insert Size (bp)2300
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MutationRemoved RSET tag
- Promoter Ef1a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer TGGAATTTGCCCTTTTTGAG
- 3′ sequencing primer GGGCCACAACTCCTCATAAA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Additional sequencing primers: Intron 2F GGGACGACATGACTTAACCAG; Intron 2R CCAGCCCTTCTCATGTTCAG; Intron 1F CCTGTATGTGACCCATGTGC; Intron 1R: GCACATGGGTCACATACAGG
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Ef1a-Con/Fon/Von GCaMP 6m was a gift from Karl Deisseroth & INTRSECT 2.0 Project (Addgene plasmid # 137165 ; http://n2t.net/addgene:137165 ; RRID:Addgene_137165) -
For your References section:
Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells of Behaving Mammals. Fenno LE, Ramakrishnan C, Kim YS, Evans KE, Lo M, Vesuna S, Inoue M, Cheung KYM, Yuen E, Pichamoorthy N, Hong ASO, Deisseroth K. Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. doi: 10.1016/j.neuron.2020.06.003. 10.1016/j.neuron.2020.06.003 PubMed 32574559