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GPKOW.240.303
(Plasmid #137582)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 137582 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pET15-MHL
  • Backbone size w/o insert (bp) 5740
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GPKOW
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    192
  • Mutation
    240-303
  • Entrez Gene
    GPKOW (a.k.a. T54, Spp2, GPATC5, GPATCH5)
  • Tag / Fusion Protein
    • (His)6-TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7F and LIC primer: ttgtatttccagggcGGGCTGGTGCCTG
  • 3′ sequencing primer T7R and LIC primer: caagcttcgtcatcaCTAATCCAAGGTGTTC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

insert+fusion tag if applicable MHHHHHHSSGRENLYFQGGLVPGGAVVVLSGPHRGLYGKVEGLDPDNVRAMVRLAVGSRVVTVSEYYLRPVSQQEFDKNTLD sequence of insert only (no fusion tag) GLVPGGAVVVLSGPHRGLYGKVEGLDPDNVRAMVRLAVGSRVVTVSEYYLRPVSQQEFDKNTLD

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GPKOW.240.303 was a gift from Tara Davis (Addgene plasmid # 137582)
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