ppih.001.177.W133A
(Plasmid #137655)

• Purpose: Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.
• Depositing Labs: Tara Davis, Melissa Jurica
• Publication: Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704. doi: 10.1042/BCJ20170366.

Backbone

• Vector backbone: pET28GST-LIC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ppih
• Species: H. sapiens (human)
• Mutation: 001-177-W133A
• Entrez Gene: PPIH (a.k.a. CYP-20, CYPH, SnuCyp-20, USA-CYP)
• Tag / Fusion Protein:
  • GST-(His)6-thrombin (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7F and LIC primer: cctgctctaagtgcgatgcgctggatgggaag
• 3′ sequencing primer: T7R and LIC primer: tgcttcccatccagcgcatcgcacttagagcagg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSSMGSSHHHHHHSSGLVPRGSMAVANSSPVNPVVFFDVSIGGQEVGRMKIELFADVVPKTAENFRQFCTGEFRKDGVPIGYKGSTFHRVIKDFMIQGGDFVNGDGTGVASIYRGPFADENFKLRHSAPGLLSMANSGPSTNGCQFFITCSKCDALDGKHVVFGKIIDGLLVMRKIENVPTGPNNKPKLPVVISQCGEM