PA-ecliptic pHluorin
(Plasmid
#137837)
-
PurposeA photoactivatable GEVI variant originated from Bongwoori-R3
-
Depositing Lab
-
Depositing OrganizationKorea Institute of Science and Technology
Located in Republic of Korea -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 137837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 137837-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 137837-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepcDNA3.1(+)
-
Backbone manufacturerThermo Fischer (Invitrogen)
- Backbone size w/o insert (bp) 5526
- Total vector size (bp) 6243
-
Modifications to backbonenone
-
Vector typeMammalian Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namePhotoactivatable-ecliptic pHluorin
-
Alt namePA-ecliptic pHluorin
-
SpeciesAequorea victoria
-
Insert Size (bp)717
-
MutationL64F, T65S, V163A, T203H
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer CMV_forward
- 3′ sequencing primer BGH_reverse
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 137837-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 137837-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PA-ecliptic pHluorin was a gift from Bradley Baker (Addgene plasmid # 137837 ; http://n2t.net/addgene:137837 ; RRID:Addgene_137837) -
For your References section:
Engineering Photoactivatability in Genetically Encoded Voltage and pH Indicators. Lee S, Song YK, Baker BJ. Front Cell Neurosci. 2019 Oct 29;13:482. doi: 10.3389/fncel.2019.00482. eCollection 2019. 10.3389/fncel.2019.00482 PubMed 31736711
