pT2ADW-lox-mCherryNLS-pA-lox-4493NES
(Plasmid #138374)

• Purpose: Booster-PKA for the generation of mice
• Depositing Lab: Michiyuki Matsuda
• Publication: Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941.

Backbone

• Vector backbone: pT2ADW
• Backbone size w/o insert (bp): 6500
• Total vector size (bp): 11242
• Modifications to backbone: pT2ADW was constructed as follows: The D4Z4 insulator was inserted before the CAG promoter of pT2AL200R175 carrying Tol2 recombination sites. Then, the WPRE sequence of pCSII-EF was inserted before the poly A sequence.
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: lox-mCherry-lox-Booster-PKA
• Species: S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 2600
• Promoter: CAG

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pCAGGS-5

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor found that the linker region of the Booster-PKA insert may be 42 bp longer than shown in their reference sequence, but that the plasmid functions as reported in the associated publication.

Sequences are based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.