PurposeBooster-PKA for the generation of mice
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||138374||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6500
- Total vector size (bp) 11242
Modifications to backbonepT2ADW was constructed as follows: The D4Z4 insulator was inserted before the CAG promoter of pT2AL200R175 carrying Tol2 recombination sites. Then, the WPRE sequence of pCSII-EF was inserted before the poly A sequence.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesS. cerevisiae (budding yeast), Synthetic
Insert Size (bp)2600
- Promoter CAG
- Cloning method Gateway Cloning
- 5′ sequencing primer pCAGGS-5 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Depositor found that the linker region of the Booster-PKA insert may be 42 bp longer than shown in their reference sequence, but that the plasmid functions as reported in the associated publication.
Sequences are based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pT2ADW-lox-mCherryNLS-pA-lox-4493NES was a gift from Michiyuki Matsuda (Addgene plasmid # 138374 ; http://n2t.net/addgene:138374 ; RRID:Addgene_138374)
For your References section:Booster, a Red-Shifted Genetically Encoded Forster Resonance Energy Transfer (FRET) Biosensor Compatible with Cyan Fluorescent Protein/Yellow Fluorescent Protein-Based FRET Biosensors and Blue Light-Responsive Optogenetic Tools. Watabe T, Terai K, Sumiyama K, Matsuda M. ACS Sens. 2020 Feb 26. doi: 10.1021/acssensors.9b01941. 10.1021/acssensors.9b01941 PubMed 32101394