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PurposeInducible expression of mCherry with nuclear localization signal
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 138527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTetOne
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4207
- Total vector size (bp) 5025
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemCherry
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SpeciesSynthetic
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Insert Size (bp)850
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GenBank IDAY678264.1
- Promoter TRE3Gs
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Tag
/ Fusion Protein
- 3x SV40 cNLS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer AACGGACGTGAAGAATGTG
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTetOne-mCherry-cNLS was a gift from Christopher Grigsby & Ana Teixeira (Addgene plasmid # 138527 ; http://n2t.net/addgene:138527 ; RRID:Addgene_138527) -
For your References section:
Modeling the transport of nuclear proteins along single skeletal muscle cells. Taylor-Weiner H, Grigsby CL, Ferreira DMS, Dias JM, Stevens MM, Ruas JL, Teixeira AI. Proc Natl Acad Sci U S A. 2020 Feb 11;117(6):2978-2986. doi: 10.1073/pnas.1919600117. Epub 2020 Jan 27. 10.1073/pnas.1919600117 PubMed 31988126
Map uploaded by the depositor.
