pXF222
              
              
                (Plasmid
                
                #139032)
              
            
            
            
          - 
            PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system
 - 
              Depositing Lab
 - 
          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139032 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
- 
            Vector backboneZero blunt TOPO
 - 
              Backbone manufacturerThermo Fisher Scientific
 - Backbone size w/o insert (bp) 3519
 - 
              Vector typeWorm Expression
 
Growth in Bacteria
- 
            Bacterial Resistance(s)Kanamycin, 50 μg/mL
 - 
            Growth Temperature37°C
 - 
            Growth Strain(s)DH5alpha
 - 
            Copy numberHigh Copy
 
Gene/Insert
- 
                Gene/Insert namemkate2 (no STOP codon)
 - 
                    SpeciesC. elegans (nematode)
 
Cloning Information
- Cloning method TOPO Cloning
 
Terms and Licenses
- 
        Academic/Nonprofit Terms
 - 
      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
- 
              
For your Materials & Methods section:
pXF222 was a gift from Erik Griffin (Addgene plasmid # 139032 ; http://n2t.net/addgene:139032 ; RRID:Addgene_139032) - 
                
For your References section:
SapTrap Assembly of Caenorhabditis elegans MosSCI Transgene Vectors. Fan X, Henau S, Feinstein J, Miller SI, Han B, Frokjaer-Jensen C, Griffin EE. G3 (Bethesda). 2019 Dec 17. pii: g3.119.400822. doi: 10.1534/g3.119.400822. 10.1534/g3.119.400822 PubMed 31848219