pHCKan-phoAp-GFPuv
(Plasmid
#139072)
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PurposeLow phosphate inducible promoter phoAp for GFP expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139072 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSMART-HC Kan
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha ; DLF_R002
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namephoAp-GFPuv
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SpeciesE. coli
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
DLF_R002 is used for recombinant protein production. It is not required to propogate the plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHCKan-phoAp-GFPuv was a gift from Michael Lynch (Addgene plasmid # 139072 ; http://n2t.net/addgene:139072 ; RRID:Addgene_139072) -
For your References section:
Media Robustness and Scalability of Phosphate Regulated Promoters Useful for Two-Stage Autoinduction in E. coli. Moreb EA, Ye Z, Efromson JP, Hennigan JN, Menacho-Melgar R, Lynch MD. ACS Synth Biol. 2020 Jun 19;9(6):1483-1486. doi: 10.1021/acssynbio.0c00182. Epub 2020 May 27. 10.1021/acssynbio.0c00182 PubMed 32353228
