cfmTurquoise-N
(Plasmid
#139284)
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PurposeCys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.
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Depositing Lab
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Depositing OrganizationFukushima Medical University
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139284 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 139284-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 139284-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonemTurquoise-N1
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Backbone manufacturerClontech
- Total vector size (bp) 4733
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecfmTurquoise
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SpeciesSynthetic
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Insert Size (bp)720
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MutationCys48Ser and Cys70/Met
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Tag
/ Fusion Protein
- Multi-cloning sites (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 139284-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 139284-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
cfmTurquoise-N was a gift from Ikuo Wada (Addgene plasmid # 139284 ; http://n2t.net/addgene:139284 ; RRID:Addgene_139284) -
For your References section:
Integrative genome analysis identified the KANNO blood group antigen as prion protein. Omae Y, Ito S, Takeuchi M, Isa K, Ogasawara K, Kawabata K, Oda A, Kaito S, Tsuneyama H, Uchikawa M, Wada I, Ohto H, Tokunaga K. Transfusion. 2019 Jul;59(7):2429-2435. doi: 10.1111/trf.15319. Epub 2019 Apr 24. 10.1111/trf.15319 PubMed 31020675
