pRC/CMV-JAK1K908E-VSV
(Plasmid
#139357)
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PurposeExpression of kinase-dead JAK1-K908E protein
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Depositing Lab
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Depositing OrganizationInstitut Pasteur
Located in France -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 139357 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 139357-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 139357-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepRC/CMV
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameJAK1 cDNA with 300nt of 5' UTR
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SpeciesH. sapiens (human)
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MutationK908E
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Entrez GeneJAK1 (a.k.a. AIIDE, JAK1A, JAK1B, JTK3)
- Promoter CMV
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Tag
/ Fusion Protein
- VSV (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AfeI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byoriginal JAK1 cDNA clone from Andrew Wilks, Ludwig Institute for Cancer Research, Victoria, Australia
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 139357-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 139357-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRC/CMV-JAK1K908E-VSV was a gift from Sandra Pellegrini (Addgene plasmid # 139357 ; http://n2t.net/addgene:139357 ; RRID:Addgene_139357) -
For your References section:
Two rare disease-associated Tyk2 variants are catalytically impaired but signaling competent. Li Z, Gakovic M, Ragimbeau J, Eloranta ML, Ronnblom L, Michel F, Pellegrini S. J Immunol. 2013 Mar 1;190(5):2335-44. doi: 10.4049/jimmunol.1203118. Epub 2013 Jan 28. 10.4049/jimmunol.1203118 PubMed 23359498
