pRC/CMV-JAK1P1084A-VSV
(Plasmid #139358)

• Purpose: Expression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)
• Depositing Lab: Sandra Pellegrini
• Publication: Li et al J Immunol. 2013 Mar 1;190(5):2335-44. doi: 10.4049/jimmunol.1203118. Epub 2013 Jan 28.

Backbone

• Vector backbone: pRC/CMV
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: JAK1 cDNA with 300nt of 5' UTR
• Species: H. sapiens (human)
• Mutation: P1084A
• Entrez Gene: JAK1 (a.k.a. AIIDE, JAK1A, JAK1B, JTK3)
• Promoter: CMV
• Tag / Fusion Protein:
  • VSV (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AfeI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: Sp6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: original JAK1 cDNA clone from Andrew Wilks, Ludwig Institute for Cancer Research, Victoria, Australia

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry