pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA
(Plasmid #139513)

• Purpose: B6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele targeting vector, low copy number
• Depositing Lab: Mario Capecchi
• Publication: Miscellaneous gene targeting vector plasmids (unpublished)

Backbone

• Vector backbone: pDTA-4
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Mouse Targeting ; Dre/rox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CAG rox stop rox td-sfGFP dre reporter
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • pHipp11-DTA-dre reporter tdGFP.gb
• A portion of this plasmid was derived from a plasmid made by: CAG promoter is from pCAGGS obtained from BCCM/LMBP. FNF is from plasmid #22687. bGH pA is from Neal Copeland. SV40 pA is from Clontech. sfGFP is from Sandia Biotech. WPRE is from Miguel Ramalho-Santos.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Homology arms subcloned by recombineering from a clone of RPCI-23 C57BL/6J mouse genomic DNA library.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.

Please note there are a few ambiguous bases in Addgene’s NGS result. This is due to repetitive G-C region. Depositor confirms these bases do not affect plasmid function.

Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.

In plasmids constructed later for this collection, the four nucleotides that were previously missing in the 5' end of the WPRE were added back, and SnapGene now recognizes it as WPRE. For example, see https://www.addgene.org/177150/.