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(Plasmid #140019)


Item Catalog # Description Quantity Price (USD)
Plasmid 140019 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    EcoLeuRS (AnapRS)
  • Alt name
    4xh7SK LeuTyrT(CUA)
  • Species
    Escherichia coli
  • Insert Size (bp)
  • Mutation
    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L538S, F541C, A560V
  • Promoter EF1

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS and depositors sanger sequencing can only confirm 3x EcoLeuT(CUA) repeats. Depositor however confirms plasmid is functional. We recommend additional diagnostic tests to confirm there are 4 tRNA repeats.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS_4xEcoLeuT(CUA)_AnapRS was a gift from Simon Elsaesser (Addgene plasmid # 140019 ; ; RRID:Addgene_140019)
  • For your References section:

    Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery. Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, Landreh M, Elsasser SJ. Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6. 10.1016/j.crmeth.2023.100626 PubMed 37935196