Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery.
Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, Landreh M, Elsasser SJ
Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6. PubMed Article
Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, Landreh M, Elsasser SJ
Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140018 | pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG | Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration |
| 140019 | pAS_4xEcoLeuT(CUA)_AnapRS | Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of azido-phenylalanine; for transient or stable piggyBac-mediated integration |
| 140020 | pAS_4xMma PylT_FLAG-AbKRS-chIPYE | Plasmid with 4xMmaPylT cassette and chimeric Mma/Mba PylRS for amber suppression and incorporation of photo-crosslinking-lysine; for transient or stable piggyBac-mediated integration |
| 140021 | pAS_4xMma PylT_FLAG-AcKRS | Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration |
| 140022 | pAS_4xMma PylT_FLAG-PcKRS | Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration |
| 174891 | pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG | amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette |
| 174892 | pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG | amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette |
| 174894 | pAS_4xEcoLeuT(CUA)_EF1_AnapRS | AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration |
| 174895 | pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA | amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette |
| 174896 | pAS_4xMmaPylT(UUA)_EF1_MmaPylRS | Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration |
| 174897 | pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA | ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette |
| 174898 | pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA | opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette |
| 174899 | pAS_4xMmaPylT(UCA)_EF1_MmaPylRS | Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration |