pAS_4xEcoLeuT(CUA)_EF1_AnapRS
(Plasmid
#174894)
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PurposeAnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration
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Depositing Lab
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Depositing OrganizationKarolinska Institutet
Located in Sweden -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174894 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 174894-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 174894-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepAS
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Backbone manufacturerAddgene #140008 based on PB510B-1 (System Bioscience)
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnapRS
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SpeciesE. coli
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Insert Size (bp)2592
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MutationL38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L538S, F541C, A560V
- Promoter EF1
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAnapRS in mammalian cells Chatterjee et al. 2013, PMID: PMC3783214
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 174894-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 174894-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAS_4xEcoLeuT(CUA)_EF1_AnapRS was a gift from Simon Elsaesser (Addgene plasmid # 174894 ; http://n2t.net/addgene:174894 ; RRID:Addgene_174894) -
For your References section:
Dual stop codon suppression in mammalian cells with genomically integrated genetic code expansion machinery. Meineke B, Heimgartner J, Caridha R, Block MF, Kimler KJ, Pires MF, Landreh M, Elsasser SJ. Cell Rep Methods. 2023 Nov 20;3(11):100626. doi: 10.1016/j.crmeth.2023.100626. Epub 2023 Nov 6. 10.1016/j.crmeth.2023.100626 PubMed 37935196
