pSW068
(Plasmid #140036)

• Purpose: KO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoter
• Depositing Lab: Peter Nixon
• Publication: Unprecedented biomass and fatty acid production by the newly discovered cyanobacterium Synechococcus sp. PCC 11901 (unpublished)

Backbone

• Vector backbone: pUC19
  (Search Vector Database)
• Total vector size (bp): 7480
• Modifications to backbone: Up and downstream homology regions for recombination into the PCC 11901 fadD locus; cLac143 promoter and LacI gene under pMB2 promoter control; Kanamycin resistance cassette
• Vector type: Bacterial Expression
• Selectable markers: Carbenicillin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Truncated thioesterase gene
• Alt name: 'tesA
• Species: Escherichia coli
• Insert Size (bp): 549
• Promoter: cLac143

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: cttttaaatggaattgcctctggacatctccaaaCGAATTGTGA
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • psw068-puc-ssw07-faddtesa-clac143.gb
• A portion of this plasmid was derived from a plasmid made by: GenScript (codon-optimization for PCC 7002)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry