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(Plasmid #140035)


Item Catalog # Description Quantity Price (USD)
Plasmid 140035 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 7582
  • Modifications to backbone
    Up and downstream sequences for homologous recombination into PCC 11901 psbA2 locus; cLac143 inducible promoter and LacI gene under pMB2 promoter; Spectinomycin resistance cassette
  • Vector type
    Bacterial Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Yellow fluorescent protein
  • Alt name
  • Species
    Synthetic; Aequorea victoria
  • Insert Size (bp)
  • GenBank ID
  • Promoter cLac143

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ccccagtactttaaactggtacatgcaattgactcccctctggacatctcca
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSW039 was a gift from Peter Nixon (Addgene plasmid # 140035 ; ; RRID:Addgene_140035)