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Salsa6f
(Plasmid #140188)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 140188 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3923
  • Total vector size (bp) 6698
  • Modifications to backbone
    The EGFP gene has been replaced with the ratiometric genetically encoded calcium indicator Salsa6f (tdTomato-V5-GCaMP6f)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Salsa6f
  • Alt name
    tdTomato-v5-GCaMP6f
  • Species
    Synthetic
  • Insert Size (bp)
    2775
  • Promoter CMV Immediate Early
  • Tag / Fusion Protein
    • tdTomato-v5-GCaMP6f

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TGACGTCAATGGGAGTTTG
  • 3′ sequencing primer ACATTGATGAGTTTGGAC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The fusion protein in this plasmid is the fluorescent genetically encoded calcium indicator Salsa6f, which is a fusion of the dimeric red fluorescent protein tdTomato, the epitope tag V5, and the green fluorescent genetically encoded calcium indicator GCaMP6f, in order from N- to C-termini. The presence of tdTomato enables visualization of transfected cells in the absence of Ca2+ signaling and concentration-independent, ratiometric readout of Ca2+ signals. Salsa6f has been introduced into transgenic mice, where its expression is under Cre recombinase control (see PMID: 29239725).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Salsa6f was a gift from Michael Cahalan (Addgene plasmid # 140188 ; http://n2t.net/addgene:140188 ; RRID:Addgene_140188)
  • For your References section:

    T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse. Dong TX, Othy S, Jairaman A, Skupsky J, Zavala A, Parker I, Dynes JL, Cahalan MD. Elife. 2017 Dec 14;6. pii: 32417. doi: 10.7554/eLife.32417. 10.7554/eLife.32417 PubMed 29239725