pAc-­His-­zfKitlga
(Plasmid #140292)

• Purpose: Express soluble His-tagged zebrafish Kitlga in insect cells
• Depositing Lab: Petr Bartunek
• Publication: Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. doi: 10.1182/bloodadvances.2020001700.

Backbone

• Vector backbone: pAcGP-67A
• Backbone size w/o insert (bp): 9748
• Total vector size (bp): 10467
• Modifications to backbone: 6xHis tag insert
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Kitlga
• Alt name: Kit ligand a
• Alt name: SCFa
• Species: D. rerio (zebrafish)
• Insert Size (bp): 486
• Entrez Gene: kitlga (a.k.a. kitla)
• Tag / Fusion Protein:
  • 6x His (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CCGGATTATTCATACCGTCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry