pN_empty
(Plasmid
#140404)
-
Purpose(Empty Backbone) Empty backbone with nourseothricin selection in plants
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 140404 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepB2GW7
-
Backbone manufacturerhttps://gateway.psb.ugent.be
- Backbone size (bp) 8179
-
Modifications to backboneBASTA resistance marker (bar) replaced with nourseothricin resistance marker (nat). 35S promoter and ccdB coding sequence removed.
-
Vector typePlant Expression, Synthetic Biology
-
Selectable markersNourseothricin/streptothricin
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pN_empty was a gift from Mathias Pribil (Addgene plasmid # 140404 ; http://n2t.net/addgene:140404 ; RRID:Addgene_140404)