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Addgene

pN_UBQ10/mApple
(Plasmid #140405)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 140405 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pN_35S/mApple
  • Backbone size w/o insert (bp) 8202
  • Total vector size (bp) 9577
  • Vector type
    Plant Expression, Synthetic Biology
  • Selectable markers
    Nourseothricin/streptothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mApple
  • Species
    Synthetic
  • Insert Size (bp)
    711
  • Promoter AtUBQ10

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TAGTTTCTAGTTTGTGCGATCG
  • 3′ sequencing primer ATATGCTCAACACATGAGCGA
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    mApple was amplified from mApple-pBAD, a gift from Michael Davidson & Nathan Shaner & Roger Tsien (Addgene plasmid # 54536 ; http://n2t.net/addgene:54536 ; RRID:Addgene_54536).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN_UBQ10/mApple was a gift from Mathias Pribil (Addgene plasmid # 140405 ; http://n2t.net/addgene:140405 ; RRID:Addgene_140405)
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