CAaNa::GFP
(Plasmid
#140507)
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PurposeEncodes GFP downstream of an attP/attB flanked Neomycin stop cassette driven by the CAG promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 140507 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCALNL-GFP
- Backbone size w/o insert (bp) 5644
- Total vector size (bp) 6927
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Vector typeMammalian Expression ; Chicken Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameattP-Neomycin-PolyA-attB
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Insert Size (bp)1283
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CTGCTAACCATGTTCATGCC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byNeomycin stop cassette from Addgene plasmid #13770
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAaNa::GFP was a gift from Mark Emerson (Addgene plasmid # 140507 ; http://n2t.net/addgene:140507 ; RRID:Addgene_140507) -
For your References section:
Lineage tracing analysis of cone photoreceptor associated cis-regulatory elements in the developing chicken retina. Schick E, McCaffery SD, Keblish EE, Thakurdin C, Emerson MM. Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.1038/s41598-019-45750-7. 10.1038/s41598-019-45750-7 PubMed 31249345