pCDNA3.1 hSCN5A-L325R
(Plasmid #145373)

• Purpose: expresses mutant form of human Nav1.5 (L325R) found in patient with Brugada syndrome in mammalian cells
• Depositing Lab: Hugues Abriel
• Publication: Keller et al Cardiovasc Res. 2005 Aug 15;67(3):510-9. doi: 10.1016/j.cardiores.2005.03.024.

Backbone

• Vector backbone: pCDNA3.1-Zeo (+)
• Backbone size w/o insert (bp): 5015
• Total vector size (bp): 11215
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sodium voltage-gated channel alpha subunit 5
• Alt name: Nav1.5
• Alt name: SCN5A
• Species: H. sapiens (human)
• Insert Size (bp): 6200
• Mutation: changed Leucine 325 to Arginine
• GenBank ID:
• Entrez Gene: SCN5A (a.k.a. CDCD2, CMD1E, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SSS1, VF1)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 Fwd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry