pCDNA3.1 hSCN5A_deltaSIV
(Plasmid #145771)

• Purpose: expresses human Nav1.5 lacking the SIV motif in mammalian cells
• Depositing Lab: Hugues Abriel
• Depositing Organization: Universite de Lausanne (UNIL)
  Located in Switzerland
• Publication: Jespersen et al Biochem Biophys Res Commun. 2006 Oct 6;348(4):1455-62. doi: 10.1016/j.bbrc.2006.08.014. Epub 2006 Aug 10.

Backbone

• Vector backbone: pCDNA3.1-Zeo (+)
• Backbone size w/o insert (bp): 5015
• Total vector size (bp): 11215
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sodium voltage-gated channel alpha subunit 5
• Alt name: Nav1.5
• Alt name: SCN5A
• Species: H. sapiens (human)
• Insert Size (bp): 6200
• Mutation: Stop codon at Serine 2013
• GenBank ID:
• Entrez Gene: SCN5A (a.k.a. CDCD2, CMD1E, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SSS1, VF1)
• Promoter: CMV
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Introduction of S2013stop mutation into plasmid pCDNA3.1 hSCN5A (ID: 145374) which was a gift from RS Kass (Columbia University, USA)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

DNA (Catalog # 145771-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 145771-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.