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pCDNA3.1 hSCN5A_deltaSIV
(Plasmid #145771)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 145771 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDNA3.1-Zeo (+)
  • Backbone size w/o insert (bp) 5015
  • Total vector size (bp) 11215
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sodium voltage-gated channel alpha subunit 5
  • Alt name
    Nav1.5
  • Alt name
    SCN5A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6200
  • Mutation
    Stop codon at Serine 2013
  • GenBank ID
  • Entrez Gene
    SCN5A (a.k.a. CDCD2, CMD1E, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SSS1, VF1)
  • Promoter CMV
  • Tag / Fusion Protein
    • none

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Introduction of S2013stop mutation into plasmid pCDNA3.1 hSCN5A (ID: 145374) which was a gift from RS Kass (Columbia University, USA)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3.1 hSCN5A_deltaSIV was a gift from Hugues Abriel (Addgene plasmid # 145771 ; http://n2t.net/addgene:145771 ; RRID:Addgene_145771)
  • For your References section:

    Cardiac sodium channel Na(v)1.5 interacts with and is regulated by the protein tyrosine phosphatase PTPH1. Jespersen T, Gavillet B, van Bemmelen MX, Cordonier S, Thomas MA, Staub O, Abriel H. Biochem Biophys Res Commun. 2006 Oct 6;348(4):1455-62. doi: 10.1016/j.bbrc.2006.08.014. Epub 2006 Aug 10. 10.1016/j.bbrc.2006.08.014 PubMed 16930557