pCI.neo-rPAM1-M314I
(Plasmid #145802)

• Purpose: expresses catalytically inactive rat PAM-1 in mammalian cells
• Depositing Labs: Betty Eipper, Richard Mains
• Publication: Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65. doi: 10.1021/bi00009a016.

Backbone

• Vector backbone: pCIS
• Backbone manufacturer: Cornelia Gorman
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 8000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase)
• Species: R. norvegicus (rat)
• Insert Size (bp): 1350
• Mutation: changed methionine 314 to isoleucine to inactivate monooxygenase activity
• GenBank ID: AAC05607.1
• Entrez Gene: Pam (a.k.a. PHM)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind3 (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: TAATACGACTCACTATAGG
• 3′ sequencing primer: GTGGTTTGTCCAAACTCATC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry