pQE9-His-p97(wt)
(Plasmid #14666)

• Depositing Lab: Graham Warren
• Publication: Meyer et al EMBO J. 2000 May 15. 19(10):2181-92.

Backbone

• Vector backbone: pQE9
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 3300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Growth instructions: Needs strain with lacIq
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: p97
• Alt name: VCP
• Alt name: valosin-containing protein
• Species: M. musculus (mouse)
• Insert Size (bp): 2415
• Mutation: See depositor comments below.
• GenBank ID: NM_009503
• Entrez Gene: Vcp (a.k.a. 3110001E05, CDC48, p97, p97/VCP)
• Tag / Fusion Protein:
  • RGS6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHi (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS results identified a V206 variant in p97 that matches NCBI reference CAA78412.1.