Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21267||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesR. norvegicus (rat)
Insert Size (bp)1824
Entrez GeneNploc4 (a.k.a. Npl4)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BglII (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET30-Npl4 was a gift from Hemmo Meyer (Addgene plasmid # 21267 ; http://n2t.net/addgene:21267 ; RRID:Addgene_21267)
For your References section:The AAA ATPase p97/VCP interacts with its alternative co-factors, Ufd1-Npl4 and p47, through a common bipartite binding mechanism. Bruderer RM, Brasseur C, Meyer HH. J Biol Chem. 2004 Nov 26. 279(48):49609-16. 10.1074/jbc.M408695200 PubMed 15371428