Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pET26-Ufd1-His
(Plasmid #21266)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21266 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET26
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Ufd1
  • Alt name
    UFD1L
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    921
  • Entrez Gene
    Ufd1l (a.k.a. Ufd1)
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

Addgene Next-generation sequencing QC processes identified that the insert harbors truncations of the region encoding the first 4 amino acids and final amino acid of Ufd1, relative to the NCBI reference sequence NM_011672

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET26-Ufd1-His was a gift from Hemmo Meyer (Addgene plasmid # 21266 ; http://n2t.net/addgene:21266 ; RRID:Addgene_21266)
  • For your References section:

    The AAA ATPase p97/VCP interacts with its alternative co-factors, Ufd1-Npl4 and p47, through a common bipartite binding mechanism. Bruderer RM, Brasseur C, Meyer HH. J Biol Chem. 2004 Nov 26. 279(48):49609-16. 10.1074/jbc.M408695200 PubMed 15371428