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human K18
(Plasmid #14741)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14741 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Keratin 18
  • Alt name
    KRT18
  • Species
    H. sapiens (human)
  • Entrez Gene
    KRT18 (a.k.a. CK-18, CYK18, K18)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Cloning information may not be accurate. Backbone could be pcDNA3.1. The plasmid was used for transfection by the Omary lab. For example, see Ku et al 2002 JBC 277:10775, Ku et al 1998 EMBO 17:1892.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    human K18 was a gift from M Bishr Omary (Addgene plasmid # 14741 ; http://n2t.net/addgene:14741 ; RRID:Addgene_14741)
  • For your References section:

    Identification of the major physiologic phosphorylation site of human keratin 18: potential kinases and a role in filament reorganization. Ku NO, Omary MB. J Cell Biol. 1994 Oct . 127(1):161-71. 10.1083/jcb.127.1.161 PubMed 7523419