Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14742||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameKeratin 8
SpeciesH. sapiens (human)
Entrez GeneKRT8 (a.k.a. CARD2, CK-8, CK8, CYK8, K2C8, K8, KO)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer pMRB101-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Cloning information may not be accurate. The plasmid was used for transfection by the Omary lab. For example, see Ku et al 2002 JBC 277:10775, Ku et al 1998 EMBO 17:1892.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:human K8 was a gift from M Bishr Omary (Addgene plasmid # 14742 ; http://n2t.net/addgene:14742 ; RRID:Addgene_14742)
For your References section:Keratin 8 phosphorylation by p38 kinase regulates cellular keratin filament reorganization: modulation by a keratin 1-like disease causing mutation. Ku NO, Azhar S, Omary MB. J Biol Chem. 2002 Mar 29. 277(13):10775-82. 10.1074/jbc.M107623200 PubMed 11788583