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HA PKB T308D S473D pcDNA3
(Plasmid #14751)

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Item Catalog # Description Quantity Price (USD)
Plasmid 14751 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 14751-D50 50 μg of DNA in Tris buffer $495 *
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DNA 14751-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PKB DD
  • Alt name
    Protein Kinase B alpha
  • Alt name
    Akt1
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    1500
  • Mutation
    T308D S473D. Constitutively active mutant.
  • Entrez Gene
    AKT1 (a.k.a. BOS_20453)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

KpnI(1934) and NheI(2429) represent T308->D and S473->D mutations respectively

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA PKB T308D S473D pcDNA3 was a gift from Jim Woodgett (Addgene plasmid # 14751 ; http://n2t.net/addgene:14751 ; RRID:Addgene_14751)

  • For your References section:

    Multiple phosphoinositide 3-kinase-dependent steps in activation of protein kinase B. Scheid MP, Marignani PA, Woodgett JR. Mol Cell Biol. 2002 Sep . 22(17):6247-60. 10.1128/MCB.22.17.6247-6260.2002 PubMed 12167717
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