|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14931||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1400
Mutationfull-length Smad2 is fused to a nuclear-localization sequence (NLS)
Entrez GeneSMAD2 (a.k.a. JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV5 Flag-NLS-Smad2 was a gift from Joan Massague (Addgene plasmid # 14931 ; http://n2t.net/addgene:14931 ; RRID:Addgene_14931)
For your References section:Ubiquitin-dependent degradation of TGF-beta-activated smad2. Lo RS, Massague J. Nat Cell Biol. 1999 Dec . 1(8):472-8. 10.1038/70258 PubMed 10587642
Map uploaded by the depositor.