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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in the United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 14932 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 14932-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 14932-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSG424
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Backbone manufacturerMark Ptashne
- Backbone size w/o insert (bp) 3400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSmad2
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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GenBank IDAF027964
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Entrez GeneSMAD2 (a.k.a. CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2)
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Tag
/ Fusion Protein
- Gal4 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
An EcoRI fragment of Human Smad2 encoding 21nts 5'UTR, the coding region of Smad2, and 50nts of 3'UTR was inserted into the EcoRI site of pSG424.
The author's map shows the empty pSG424 map, which was described in Sadowski and Ptashne (1989) Nucleic Acids Research Vol 17 (18): p7539.
DNA (Catalog # 14932-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 14932-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG424 Smad2 (Gal4-Smad2) was a gift from Joan Massague (Addgene plasmid # 14932 ; http://n2t.net/addgene:14932 ; RRID:Addgene_14932) -
For your References section:
Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes. Liu F, Pouponnot C, Massague J. Genes Dev. 1997 Dec 1. 11(23):3157-67. 10.1101/gad.11.23.3157 PubMed 9389648
Map uploaded by the depositor.
