XF1 GAL Smad3
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12635||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneXF1 Gal
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1275
Entrez GeneSMAD3 (a.k.a. HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3)
/ Fusion Protein
- Gal4 DBD (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer Gal4 forward (Common Sequencing Primers)
Terms and Licenses
The Gal4 DNA Binding Domain (aa1-147) was fused to full-length Smad3. See author's map for details.
Also see: Feng et al (1998) Genes and Development 12, 2153-2163.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:XF1 GAL Smad3 was a gift from Rik Derynck (Addgene plasmid # 12635 ; http://n2t.net/addgene:12635 ; RRID:Addgene_12635)
For your References section:Smad3 and Smad4 cooperate with c-Jun/c-Fos to mediate TGF-beta-induced transcription. Zhang Y, Feng XH, Derynck R. Nature. 1998 Aug 27. 394(6696):909-13. 10.1038/29814 PubMed 9732876
Map uploaded by the depositor.