pSG424 Smad2 (Gal4-Smad2)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14932||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerMark Ptashne
- Backbone size w/o insert (bp) 3400
Vector typeMammalian Expression
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)1500
Entrez GeneSMAD2 (a.k.a. JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2)
/ Fusion Protein
- Gal4 (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
An EcoRI fragment of Human Smad2 encoding 21nts 5'UTR, the coding region of Smad2, and 50nts of 3'UTR was inserted into the EcoRI site of pSG424.
The author's map shows the empty pSG424 map, which was described in Sadowski and Ptashne (1989) Nucleic Acids Research Vol 17 (18): p7539.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSG424 Smad2 (Gal4-Smad2) was a gift from Joan Massague (Addgene plasmid # 14932 ; http://n2t.net/addgene:14932 ; RRID:Addgene_14932)
For your References section:Dual role of the Smad4/DPC4 tumor suppressor in TGFbeta-inducible transcriptional complexes. Liu F, Pouponnot C, Massague J. Genes Dev. 1997 Dec 1. 11(23):3157-67. 10.1101/gad.11.23.3157 PubMed 9389648
Map uploaded by the depositor.